文章编号] 1674-4721（2018）4（c）-0009-05
The relationship of myocardial apoptosis and endoplasmic reticulum stress-related protein CHOP in swine following coronary microembolization
LIN Chong-qiang1 LIU Tao2 TANG Zhong-li3 LIU Yang-chun4 LI Lang4
1.Department of Cardiovasolar Medicine，People′s Hospital of Cenxi City in Guangxi Zhuang Autonomous Region，Cenxi 543200，China；2.Department of Cardiovasolar Medicine，Minzu Hospital of Guangxi Zhuang Autonomous Region，Nanning 530001，China；3.Department of Cardiovasolar Medicine，People′s Hospital of Dao County in Hunan Province，Dao County 425300，China；4.Department of Cardiology，the First Affiliated Hospital of Guangxi Medical University，Nanning 530001，China
[Abstract]Objective To investigate the relation of myocardial apoptosis and endoplasmic reticulum stress-related （CME） protein C/EBP homologous protein （CHOP） in swine following coronary microembolization.Methods Fifty swine were selected and randomly divided into the Sham group （n=25） and the CME group （n=25）.Five subgroups were assigned according to time points （3，6，12，24，48 h），5 cases in each group.The CME model was established through injecting microspheres into the coronary artery，while swine in the Sham group received the same dose of normal saline instead.Cardiac function was measured with echocardiography.Histopathological changes were detected with HE.Microinfarction and apoptotic index were detected with hematoxylinbasic fuchsinpicric acid （HBFP） and TUNEL stainings，respectively.The expressions of CHOP and Caspase-3 proteins were determined with Western blot.Results Compared with the Sham groups，the cardiac function was decreased in varying degrees in the corresponding CME groups （P<0.05）.The cardiac function of 12 h following CME was the worst.Microinfarction rather than massive necrosis was found each time points following CME，mainly located in left ventricle and subendocardial myocardium.The difference，however，was not statistically significant （P>0.05）.Compared with the Sham groups，the apoptotic index in the corresponding CME groups were increased in varying degrees （P<0.05）.It peaked 12 h following CME.Apoptotic cardiomyocytes were mainly located in microinfarct area and its border area.Compared with the Sham groups，CHOP protein expression in the corresponding groups following CME enhanced in varying degrees （P<0.05），showing dynamic changes.It peaked 12 h following CME.Conclusion CME induces cardiomyocyte apoptosis and cardiac dysfunction，in which enhanced CHOP and caspase-3 expression may be involved.
